In January 2024, a study led by Professor Bing Liu from Fifth Medical Center, Medical Innovation Research Department, Chinese PLA General Hospital was published in the international academic journal ——Blood Science . The title of the study is "Hematopoietic stem cell heterogeneity in non-human primates revealed by five-lineage output bias analysis". This study elucidates the intricate dynamics of hematopoietic stem cell (HSC) diversity and lineage output bias in non-human primates (NHPs), providing insights valuable for both basic research and clinical applications.
Hematopoietic stem cells (HSCs) are pivotal in maintaining blood cell production, and their diversity in lineage output bias significantly influences hematopoietic processes and therapeutic strategies for blood disorders.
This study investigates HSC behavior post-transplantation in non-human primates (NHPs), revealing six distinct HSC subtypes with dynamic lineage output biases. Multi-faceted, incorporating sophisticated techniques were employed in this study to decipher the intricacies of HSC behavior post-transplantation. Hierarchical clustering techniques were utilized to identify distinct HSC subtypes based on their lineage output preferences. This cutting-edge technology allows for the unbiased classification of HSCs into meaningful groups, laying the groundwork for subsequent analysis. The selection of 820 HSC clones capable of reconstituting all major blood lineages ensured a representative sample for investigating lineage output biases. Furthermore, lineage bias scoring methods were employed to quantitatively assess the extent of lineage output bias within each HSC subtype. These findings enrich our understanding of HSC heterogeneity and provide potential implications for basic research and clinical applications in other cell types and organs.
This study advances our understanding of HSC heterogeneity and lineage output bias in NHPs, offering insights into the adaptive capabilities of HSCs in hematopoietic homeostasis. The identification of diverse HSC subtypes and their temporal evolution post-transplantation has implications for basic research and clinical applications. The refined classification method provides a robust framework for characterizing HSC diversity, aiding the development of targeted therapies for hematological disorders. These findings underscore the importance of NHP models in advancing our understanding of human hematopoiesis and highlight the translational potential of HSC research in NHPs.
Reference：Zhang, Man,et al. Hematopoietic stem cell heterogeneity in non-human primates revealed by five-lineage output bias analysis. Blood Sci. 2024 Jan 10;6(1):e00176.
